Fig 2: The inflammatory response is reduced after inhibition of ROS generation. (A) The mRNA expression levels of inflammasome pathway-related genes in each group were determined by qPCR (n = 3). (B) The protein expression levels of inflammasome pathway-related genes in each group were determined by WB (n = 3). *p < .05, **p < .01. The data are presented as the mean ± SD. siRNA: small interfering RNA; ROS: reactive oxygen species; sPLA2-IB: secretory phospholipase A2 group IB, a ligand for PLA2R.

Fig 3: Overexpressed PLA2R aggravates podocyte pyroptosis. (A) The pyroptosis level of podocytes in different groups was determined by FLICA/PI double staining and flow cytometry (n = 3). (B) Changes in podocytes in each group were observed under an electron microscope, and the arrows indicate autophagolysosomes. **p < .01. The data are presented as the mean ± SD. NC: negative control; siRNA: small interfering RNA; aas: autophagolysosomes; sPLA2-IB: secretory phospholipase A2 group IB, a ligand for PLA2R.